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KMID : 0861020030180010133
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Korea Journal of Herbology
2003 Volume.18 No. 1 p.133 ~ p.144
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The morphological standard and isolation and structure elucidation of radical scavengers from Chrysanthemum indicum L.
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徐明源/Seo, Myung-Won
鄭昇溢/申喆均/朱榮承/Jeong, Seung-Il/Shin, Chol-Gyun/Ju, Young-Sung
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Abstract
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Objectives : The purpose of the study is to enhance the objectivity and availability of the oriental herb medicine by formulating the morphological standard and isolation and structure elucidation of radical scavengers from Chrysanthemum indicum L. These intents are expected to be useful in the examination of several physiochemical and biological reactions, determination of indicator material, and identification of gene with basic sources in the future.
Methods : In order to develop the standard formulas and isolation and structure elucidation of radical scavengers from Chrysanthemum indicum L., the experimental examinations of the original herbs have been carried out. The morphological standard was divided into 4 parts as plant · external · internal · powder shape. Three antioxidative compouds were isolated by a bioassay using a 1, 1-diphenyl-2-picrydrazyl(DPPH) free radical. They were identified as luteolin, apigenin and linarin (acacetin-7-O-rutinoside) on the basis of 1H and 13C-NMR and MS data.
Results and Conclusions :
1. The plant shape :
The color of the slender-hair in its stem is purple-black. The leaf is oval-orbicular shape and has the slender-hair in both sides and its petiole has pseudo-stipule. The entire diameter of flower is 2~2.5cm. The lingulate flower is yellow and the siphonoic flower is deep-yellow color.
2. The external shape of herb:
It is a globular-like type, 2~3cm in diameter and brown-yellow to yellow color. The lingulate flower is yellow-white color haved one layer and the siphonoic flower is deep-yellow color. The external layer is oval-velvet and the internal layer is long-oval shape in the bracteole.
3. The internal shape of herb:
The change of the petal is relatively large, the upper epidermal cell is irregular oval or sphere shape and the lower epidermal cell is regular sphere shape. The lower epidermal cell has occasionally trichomes or a few air pores. the mesophyll tissue has small thin-walled cells which are relatively dense, and the secretory tissue and reserve substances of the pipe type. The vascular bundle which has reticulate vessel is distributed near to lower epidermis cells.
4. The powder shape of herb:
The color of the powder sample is yellow-brown. The fragments of parenchyma, vessel and the calcium oxalate crystal are observed. In the sample of maceration, the epidermal cell of petal is various, the epidermal cell of filament is many side shape. The parenchyma is mostly oval shape which occasionally concludes the calcium oxalate crystal. The vessel has uniforation plate at each edge, which is a spiral vessel, and the type of the pit of lateral wall is alternate shape
5. Isolation and Structure Elucidation of Radical Scavengers:
The DPPH radical scavenging activity (RC_50: 13.3㎍) of apigenin was similar to that of α-tocopherol (12.1㎍) and L-ascobic acid (9.8㎍).
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